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Comparison of Levels of Cytochromes P‐450, CYP1A2, CYP2E1, and Their Related Monooxygenase Activities in Human Surgical Liver Samples
Author(s) -
Lucas Danièle,
Berthou François,
Dréano Yvonne,
Lozac'h Patrick,
Volant Alain,
Ménez JeanFrançois
Publication year - 1993
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1993.tb00861.x
Subject(s) - cyp1a2 , cyp2e1 , chlorzoxazone , monooxygenase , hydroxylation , chemistry , cytochrome , microsome , medicine , biochemistry , gastroenterology , cytochrome p450 , metabolism , enzyme
Hepatic microsomal cytochromes P‐450 CYP1A2, and CYP2E1 contents and catalytic activities have been simultaneously investigated in 42 patients undergoing diagnostic liver biopsy. CYP1A2 contents, measured by Western blotting, were correlated with methoxyresorufin‐O‐demethylation and ethoxyresorufin‐O‐deethylation ( r = 0.65 and r = 0.66, p < 0.001, respectively). CYP2E1 contents were correlated with 1‐butanol oxidation and 6‐hydroxylation of chlorzoxazone ( r = 0.75 for both, p < 0.001). CYP1A2 catalytic activities varied by 30‐ to 40‐fold, whereas CYP2E1 activities varied by 6‐ to 20‐fold. In our study, these variations were not related to liver diseases or cancer of the digestive tract nor to alcohol drinking or smoking habits, because patients were alcohol‐ and tobacco‐free for 1 month before the study. Other environmental factors, diet habits, and/or genetic factors could explain the large interindividual variations observed.