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Membrane Biogenesis in the Presence of Ethanol
Author(s) -
Slomiany Amalia,
Grabska Maria,
Grzelinska Ewa,
Yamaki Kenichiro,
Kasinathan Chinnaswamy,
Slomiany Beatrix A.,
Slomiany Bronislaw L.
Publication year - 1992
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1992.tb00712.x
Subject(s) - vesicle , golgi apparatus , endoplasmic reticulum , microbiology and biotechnology , biochemistry , chemistry , biology , intracellular , membrane
To investigate the effect of ethanol on the intracellular transport in gastric epithelial cells, the in vitro system, generating transport vesicles which transfer mucus glycoprotein apopeptide (apomucin) from rough endoplasmic reticulum (RER) to Golgi, was assembled. The vesicles, generated from gastric mucous cell RER microsomes and labeled with [ 3 H]palmitic acid, were isolated from the maternal RER and characterized. The electron microscopy revealed that these RER products consisted of 80 to 100 nm smooth membrane vesicles, while the immunochemical analyses showed that they contain apomucin but were devoid of the characteristic integral proteins of the RER membrane. Incubation of apomucin transporting vesicles with Golgi in the presence of UDP‐[ 3 H]galactose resulted in the formation of glycosylated mucin and fusion of the vesicles with Golgi. Formation of ER transport vesicles was dependent on the supply of lipid precursors, and the activity of phosphoglyceride and sphingolipid synthesizing enzymes. In the presence of 60 and 120 MM ethanol, the vesicles were formed, but their lipid composition was modified. The results suggest that ethanol‐induced membrane alterations are initiated at the early stages of the membrane biogenesis and are first reflected in the lipid composition of the intracellular transport vesicles.