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Quantitation of β‐Sitosterol in Bourbon
Author(s) -
Rosenblum E. R.,
Thiel D. H.,
Campbell I. M.,
Gavaler J. S.
Publication year - 1991
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1991.tb01856.x
Subject(s) - chromatography , phytoestrogens , chemistry , gas chromatography–mass spectrometry , mass spectrometry , medicine , estrogen
Four phytoestrogens have previously been isolated and identified in alcoholic beverages. Before studies to evaluate the degree of estrogenicity of these substances can be performed, it is necessary to determine appropriate doses to be administered to experimental animals. Because β‐sitosterol is the most plentiful, being present in bourbon at μg/dl quantities, we have chosen to begin our quantitation work with this compound. The variability of the amount of β‐sitosterol contained in various brands of bourbon and in different lots of the same brand of bourbon have been assessed using combined gas chromatography/mass spectrometry (GC/MS) for quantitation following a simplified procedure to extract compounds of interest from bourbon.