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Differential Effects of Norepinephrine on Phosphatidylinositol 4,5‐Bisphosphate Stimulated Hydrolysis in Brains of Mice Genetically Selected for Differences in Ethanol Sensitivity
Author(s) -
Weiner Eric A.,
French Thomas A.,
Baker Rodney C.,
Masserano Joseph M.
Publication year - 1990
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1990.tb01834.x
Subject(s) - endocrinology , medicine , locus coeruleus , norepinephrine , cerebellum , phosphatidylinositol , chemistry , cerebral cortex , central nervous system , pons , hippocampus , biology , dopamine , biochemistry , signal transduction
The effects of norepinephrine on phosphoinositide turnover were evaluated in five brain regions of the long sleep (LS) and short sleep (SS) mice. These mice were selectively bred for differences in central nervous system sensitivity to ethanol with the LS exhibiting much greater sensitivity to a hypnotic dose of ethanol than the SS, as determined by the ability of the mice to regain their righting reflex. Norepinephrine (10 ‐3 M, 10 ‐4 M, and 10 ‐5 ) significantly increased phosphoinositide turnover in the hippocampus, hypothalamus, locus ceruleus, cerebellum, and cortex within each line of mice. Basal and norepinephrine‐stimulated phosphoinositide turnover were significantly higher in the SS mice as compared with the LS mice in the cerebellum and cortex but not the other brain regions. Incorporation of 3 H‐inositol into 3 H‐phosphatidylinositols was not different between SS and LS mice in the cerebellum and cortex. The greater norepinephrine‐stimulated phosphoinositide turnover in the cerebellum and cortex of the SS versus the LS mice may contribute to the CNS sensitivity to ethanol in these two lines of mice. However, ethanol (500 mM) had no effect on basal or norepinephrine‐stimulated phosphoinositide turnover in any of the five brain areas examined in the LS and SS mice.