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Detection of a New Acetaldehyde‐Induced Hemoglobin Fraction HbA 1ach by Cation Exchange Liquid Chromatography
Author(s) -
Sillanaukee Pekka,
Koivula Timo
Publication year - 1990
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1990.tb01825.x
Subject(s) - acetaldehyde , incubation , hemoglobin , chemistry , chromatography , fraction (chemistry) , hemoglobin a , biochemistry , ethanol
We developed a sensitive cation exchange liquid chromatographic method for analysis of acetaldehyde binding with hemoglobin. When human hemolysates were incubated in vitro with micromolar concentrations of acetaldehyde without reducing agents, HbA o was found to form two new fractions. One of them was eluated with HbA 1c , whereas a novel, previously undescribed fraction was detected between HbF and HbA 1c . This new fraction was termed HbA 1sch . The mean within‐assay variation for the different hemoglobin fractions varied from 0.45 (HbA 0 ) to 8.2% (HbA 1ach ) and the between‐assay variation from 0.6 to 19.3%, respectively. After incubation with [1,2 14 C]acetaldehyde the specific radioactivity of HbA 1ach was about 10 times higher than that of HbA 0 and twice as high as the activity of the HbA 1c fraction. The reaction kinetics of the formation of hemoglobin adducts were studied in vitro by incubation of hemolysates with various acetaldehyde concentrations. Acetaldehyde caused both short‐term and permanent changes in hemoglobin. Even low acetaldehyde concentrations from 10 to 100 μmol/liter caused detectable changes in hemoglobin. During incubation the amount of HbA 1ach and HbA 1c fractions increased rapidly within the first 10 min and decreased slowly during the next hr but did not return to the initial levels. This suggests that only the part of binding of acetaldehyde with hemoglobin is irreversible and the changes can be already detected after incubation with physiological acetaldehyde concentrations.