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Effects of Alcohol on the Import of Aldehyde Dehydrogenase Precursor into Rat Liver Mitochondria
Author(s) -
Wang Thomas T. Y.,
Pak Youngmi K.,
Weiner Henry
Publication year - 1990
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1990.tb01209.x
Subject(s) - aldehyde dehydrogenase , ethanol , mitochondrion , alcohol dehydrogenase , aldehyde , biochemistry , incubation , chemistry , alcohol , oleic acid , aldh2 , enzyme , branched chain alpha keto acid dehydrogenase complex , dehydrogenase , microsome , catalysis
We previously showed that incubation of rat liver motochondria with alcohols resulted in the inhibition of the import of aldehyde dehydrogenase precursor but not that of ormithine transcarbamylase precursor (Wang TTY, Farrés J, and Weiner H: Arch Biochem Biophys 272:440–449, 1989). The time required for inhibition of import to occur was now measured with ethanol (200 mM) and butanol (100 mM) at 0° and 30°C. It required approximately 30 min to achieve 50% inhibition with butanol and 50 min with ethanol. To further substantiate the membrane perturbing effects of alcohols, we also examined the effect of oleic acid on import. We found that incubation of mitochondria with oleic acid (0–100 μM) resulted in inhibition of aldehyde dehydrogenase precursor import in a dose response fashion. In addition to in vitro effects of alcohols on import, we conducted a preliminary study on import of protein into liver mitochondria isolated from rats fed ethanol. We found that the rate of aldehyde dehydrogenase precursor import into liver mitochondria isolated from ethanol fed rats was identical to that from control. The results are consistent with finding that the activity and amount of aldehyde dehydrogenase was the same in mitochondria isolated from the alcohol‐fed or control animals.