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Nucleotide Sequence of the ADH2 3 Gene Encoding the Human Alcohol Dehydrogenase β3 Subunit
Author(s) -
Carr Lucinda G.,
Xu Yiling,
Ho WeiHsien,
Edenberg Howard J.
Publication year - 1989
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1989.tb00383.x
Subject(s) - alcohol dehydrogenase , sequence (biology) , gene , protein subunit , nucleic acid sequence , genetics , microbiology and biotechnology , nucleotide , encoding (memory) , nadh dehydrogenase , biology , chemistry , alcohol , biochemistry , neuroscience
All nine exons of the ADH2 3 allele, which encodes the human alcohol dehydrogenase β3 subunit, have been cloned and sequenced. Comparison of this sequence to the ADH2 1 nucleotide sequence revealed only a single difference that results in an amino acid change, thus proving that the significant kinetic differences between these two isozymes is due to the Cys for Arg substitution at position 369. There are also two silent polymorphisms, and two changes in noncoding regions.