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Hepatic Synthesis of Apoproteins of Very Low Density and High Density Lipoproteins in Perfused Rat Liver: Influence of Chronic Heavy and Moderate Doses of Ethanol
Author(s) -
Lakshman M. R.,
Chirtel Stuart J.,
Chambers Laura C.,
Campbell Barbara S.
Publication year - 1989
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1989.tb00377.x
Subject(s) - medicine , endocrinology , very low density lipoprotein , ethanol , chemistry , cholesterol , body weight , metabolism , high density lipoprotein , low density lipoprotein , lipoprotein , biology , biochemistry
The effects of 6 weeks of heavy and moderate ethanol feeding to rats upon lipids and lipoprotein metabolism were determined. As compared to the control group, the heavy ethanol feeding resulted in the following changes: liver weight/kilogram body weight increased by 48% ( p < 0.001) with a concomitant 52% increase ( p < 0.001) in liver protein/kilogram body weight and a 2.75‐fold ( p < 0.001) increase in liver total lipids/kilogram body weight. in contrast, liver DNA/kilogram body weight or per liver was not affected significantly. Plasma cholesterol and triglycerides were higher by 53% ( p < 0.01) and 77% ( p < 0.01), respectively. Liver cholesterol and triglycerides were 4.4‐fold and 3.6‐fold higher ( p < 0.001), respectively. Plasma total A, was 1.72‐fold higher ( p < 0.001), whereas there was no significant difference in plasma apo E levels between the two groups. However, plasma high density lipoproteins (HDI) apo E was 48% lower ( p < 0.02) while the very low density lipoproteins (VLDL) E was 2.15‐fold higher ( p < 0.02). Hepatic total protein synthetic rate in the ethanol group was not significantly different from the control group. In contrast, labeled leucine incorporation into the total secretory proteins was inhibited by 36% ( p < 0.01) in ethanol‐fed group. Specifically, inhibitions of the synthetic rates of various secretory proteins in the ethanol group compared to the control group were as follows: by 55% ( p < 0.005) for total VLDL apoproteins, by 44% ( p < 0.05) for apo A 1 protein, by 55% ( p < 0.001) for total apo E proteins, by 62% ( p < 0.001) for VLDL apo E, by 52% ( p < 0.001) for HDL apo E and by 50% ( p < 0.001) for transferrin. In contrast, moderate ethanol feeding for six weeks did not alter any of the above parameters. Thus, impaired synthesis/secretion of VLDL and HDL could be the major cause for fatty liver after alcohol abuse.