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Effect of Ethanol and Vitamin B 6 Deficiency on Pyridoxal 5‐Phosphate Levels and Fetal Growth in Rat
Author(s) -
Lin Grace WJ.
Publication year - 1989
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1989.tb00319.x
Subject(s) - ethanol , endocrinology , medicine , fetus , gestation , fetal alcohol syndrome , pyridoxal , liquid diet , vitamin , calorie , sucrose , chemistry , alcohol , pregnancy , biology , biochemistry , phosphate , genetics
The effects of chronic ethanol consumption and dietary vitamin B 6 levels on tissue pyridoxal 5‐phosphate (PLP) contents and rat fetal development were investigated. Pregnant Sprague‐Dawley rats were given 35% ethanol‐calorie liquid diet with either adequate B 6 (1.7 mg/liter) or deficient B 6 (0.17 mg/liter), ad libitum, from gestation days 7 to 21. Control groups (adequate control and deficient control) were pair‐fed with isocaloric sucrose substituted for ethanol. Rats were killed on gestation day 21. Ethanol groups had smaller fetuses than control groups, regardless of their dietary B 6 levels. However, in B 6 deficiency, ethanol affected fetal weight more severely than in B 6 adequate state. Tissue PLP levels were determined by radioen‐zymatic method. In B 6 deficiency, ethanol feeding reduced maternal liver PLP by 22%. PLP in other tissues were not affected by ethanol. These results confirmed that chronic alcohol consumption affected fetal growth and also provided evidence that B 6 deficiency exacerbated ethanol effect.