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Investigation of an Acetaldehyde‐Hemoglobin Adduct in Alcoholics
Author(s) -
Stockham Teri L.,
Blanke Robert V.
Publication year - 1988
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1988.tb01339.x
Subject(s) - acetaldehyde , adduct , hemoglobin , chemistry , ethanol , in vitro , biochemistry , chromatography , organic chemistry
It has been proposed that an ethanol‐related adduct is formed with hemoglobin in chronic alcoholics. This proposal is based on the observation that alcoholics were found to have an elevation of minor hemoglobins but normal amounts of glycosylated hemoglobin (HbA 1c ). Since acetaldehyde is believed to be the primary candidate involved in adduct formation, investigation was focused on this particular compound. This study describes the attempt to isolate and purify an acetaldehyde‐hemoglobin adduct in alcoholics by cationexchange chromatography and m ‐aminophenylboronic acid affinity chromatography for the purpose of characterization. Using acetaldehyde concentrations of 5 and 10 mM, an acetaldehyde adduct with hemoglobin was formed in vitro, isolated, and characterized. However, a similar adduct could not be isolated in vitro using a pharmacological concentration of 100 μM acetaldehyde nor could this adduct be isolated in human alcoholic subjects.