Premium
Disturbances in Na + Transport Systems Induced by Ethanol in Human Red Blood Cells
Author(s) -
Coca Antonio,
Garay Ricardo
Publication year - 1988
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1988.tb00238.x
Subject(s) - cotransporter , bumetanide , chemistry , ouabain , ethanol , efflux , ion transporter , sodium , red blood cell , membrane transport , biophysics , stimulation , diaphragm pump , biochemistry , endocrinology , membrane , biology , materials science , organic chemistry , micropump , nanotechnology
The effects of ethanol on fluxes catalyzed by four Na + transport systems (ouabain‐sensitive Na + , K + pump, bumetanide‐sensitive Na + , K + cotransport system, Na+:Li + ‐ countertransport and anion carrier) and on Na + and K + leaks were investigated in human red blood cells. Ethanol concentrations higher than 32 my were required in order to significantly modify erythrocyte Na + transport function. The observed changes can be summarized as follows: (a) stimulation of Na + efflux through the Na + , K + pump (by 21–32% at 160–400 mm) and Na + :Li + countertransport (by 34–59% at 160–400 mmk (b) inhibition of outward Na + , K + cotransport (by 23–34% at 160–400 mm) and LiCO 3 ‐ influx through the anion carrier (by 17–21% at 64–400 mm); and (c) increase in Na + and K+ leaks (by 13–16% at 64–400 mm). The effects of ethanol on the Na+, K + pump and Na + , K + cotransport system resulted from changes in maximal rates of Na + efflux (increased and decreased, respectively) without any significant effect on the apparent affinities for internal Na + . Erythrocytes preincubated for 1 hr with 160 mm ethanol, washed and further incubated in flux media, recovered a normal Na + transport function. In conclusion, high concentrations of ethanol induced reversible perturbations of fluxes catalyzed by erythrocyte Na + transport systems. The observed effects may reflect disturbances in Na+ transport function associated with severe intoxication.