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Use of Nuclear Magnetic Resonance Spectroscopy to Study the Effects of Ethanol Consumption on Liver Metabolism and Pathology
Author(s) -
Cunningham Carol C.
Publication year - 1986
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1986.tb05084.x
Subject(s) - metabolism , nuclear magnetic resonance spectroscopy , ethanol , chemistry , biochemistry , nuclear magnetic resonance , organic chemistry , physics
The application of NMR spectroscopy to studies of intact organs represents a powerful technology applicable for investigations of alcohol‐induced liver disease in animal models. NMR‐assisted studies of ethanol metabolism and its related pathology are now possible utilizing the 13 C and 31 P nuclei primarily. The major advantage of the technology includes the capacity to investigate biochemical processes at the level of the intact organ which is analyzed as a perfused preparation or in situ in an anesthetized animal model. Quantitative measurements of compounds containing either 31 P or 13 C nuclei are possible, and the kinetics of precursor incorporation into compounds associated with carbohydrate, lipid, and amino acid metabolism are readily followed. The concentrations of free Mg 2+ and the pH of the intact liver can be monitored as a function of external perturbation and/or metabolic disturbances.