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Analysis of Human Alcohol‐ and Aldehyde‐Metabolizing Isozymes by Electrophoresis and Isoelectric Focusing
Author(s) -
Duley John A.,
Harris Owen,
Holmes Roger S.
Publication year - 1985
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1985.tb05747.x
Subject(s) - isozyme , isoelectric focusing , aldehyde dehydrogenase , alcohol dehydrogenase , biochemistry , cytosol , chemistry , aldh2 , aldehyde oxidase , enzyme , granule (geology) , isoelectric point , biology , xanthine oxidase , paleontology
Isoelectric focusing and electrophoresis were used to identify the various isozymes of alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH), aldehyde oxidase (AOX), and xanthine oxidase (XOX). ADH types I, II, and III were located primarily in the cytosol fraction of liver, but some activity was found also in the small granule fraction. The ALDH‐I and ‐IV isozymes were found in the large granule fraction, while ALDH‐II and ‐III were present in the cytosol and ALDH‐V in the small granule fraction. AOX and XOX each appeared as a single cytosolic form with some small granule activity. The tissue distribution of these isozymes is presented and the physiological role of each enzyme is discussed.