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Antibodies to Cytokeratin Filaments in Patients with Alcoholic Liver Disease
Author(s) -
Kurki Pekka,
Virtanen Ismo,
Lehto VeliPekka,
Atfthan Olof,
Salaspuro Mikko
Publication year - 1984
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1984.tb05841.x
Subject(s) - cytokeratin , autoantibody , iif , antibody , intermediate filament , alcoholic liver disease , immunofluorescence , immunology , pathology , in vivo , medicine , chemistry , biology , cytoskeleton , immunohistochemistry , cell , biochemistry , cirrhosis , microbiology and biotechnology
Previous studies have suggested that cytosketetal elements in an altered form in vivo (alcoholic hyalin, AH) are targets for an autoimmune reaction in alcoholic liver disease (ALD). In this study we assayed autoantibodies to cytosketetal cytokeratin filaments, intermediate filaments of epithelial cells, by indirect immunofluorescence technique (IIF) using cultured human amnion epithelial ceils and PtK 2 ceils as targets. Sera from 20 patients with ALO, 38 patients with urogenital cancer, 22 patients with renal diseases, and from 18 healthy controls were studied Patients with ALO had a significantly increased prevalence of cytokeratin filament autoantibodies using both PtK 2 cells (69%) and human amnion epithelial ceils (69%) as substrate, as compared to other groups. The antibodies were mainly of IgA and IgM class and could be demonstrated in all forms of ALD. Interestingly, cytoskeieton antibodies have previously been observed in diseases of viral and “autoimmune” origin