Premium
Effect of Ethanol on [ 3 H]Nitrendipine Binding to Calcium Channels in Brain Membranes
Author(s) -
Greenberg David A.,
Cooper Edward C.
Publication year - 1984
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1984.tb05732.x
Subject(s) - nitrendipine , membrane , calcium , ethanol , chemistry , biophysics , voltage dependent calcium channel , biochemistry , biology , organic chemistry
We examined the effect of ethanol on [ 3 H]nitrendipine binding to ret brain membranes, to investigate the possibility that voltage‐dependent calcium channels are involved in synaptic actions of ethanol. Ethanol inhibited specific [ 3 H]nitrendipine binding with K 1 = 460 mM, by decreasing binding affinity without altering the maximal number of sites labeled. At a lower concentration (100 mM), ethanol had no effect on inhibition of binding by verapamil or diltiazem. The high concentrations of ethanol required to produce alterations in [ 3 H] nitrendipine binding suggest that ethanol and classical calcium channel antagonists influence calcium‐mediated synaptic processes by separate mechanisms.