Premium
Lymphocyte Subsets Studied with Monoclonal Antibodies in Liver Tissues of Patients with Alcoholic Liver Disease
Author(s) -
Si Lusheng,
Whiteside Theresa L.,
Schade Robert R.,
Thiel David Van
Publication year - 1983
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1983.tb05501.x
Subject(s) - cytotoxic t cell , alcoholic liver disease , monoclonal antibody , t lymphocyte , fatty liver , biology , lymphocyte , pathology , immunology , antigen , antibody , medicine , disease , cirrhosis , in vitro , biochemistry
In order to determine the nature of T lymphocytes accumulating in hepatic tissues of patients with alcoholic liver disease, we phenotyped these cells in situ, using monoclonal antibodies to lymphocyte surface antigens and the avidin‐biotin‐peroxidase complex (ABC) technique. In portal areas of cirrhotic livers, a significant increase ( p < 0.001) in T lymphocytes was observed as compared to livers showing only fatty changes and normal her tissues. The T cells accumulating in cirrhotic livers comprised T helper‐inducer (T4) and T suppressor‐cytotoxic (T8) subsets with the T4/T8 ratio of 1.4 f 0.5 (SD, n = 14). In contrast, the T lymphocytes in the cellular projections which extended into the parenchyma consisted mostly of suppressor‐cytotoxic cells. The relative enrichment in T8+ lymphocytes among the cells invading the parenchyma suggests that they may be important in the mediation of a cytotoxic injury of hepatocytes in advanced alcoholic liver disease and in regulating an immunologic challenge possibly initiated in response to alcoholic hyaline.