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Effects of Ethanol and Temperature on Glucose Utilization in the In Vivo and Isolated Perfused Mouse Brain
Author(s) -
Towell John F.,
Erwin V. Gene
Publication year - 1982
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1982.tb05388.x
Subject(s) - in vivo , ethanol , carbohydrate metabolism , perfusion , cerebellum , pons , medicine , endocrinology , chemistry , glucose uptake , biology , biochemistry , insulin , microbiology and biotechnology
A [ 14 C]2‐deoxy‐ d ‐glucose technique was used to investigate the effects of ethanol and temperature variation on the rate of glucose utilization in the isolated perfused mouse brain. Glucose utilization rates in six regions of the isolated perfused mouse brain at 37°C were similar to the rates in comparable regions of the in vivo mouse brain. The temperature dependence of the glucose utilization rate in the perfused mouse brain suggests that a change in the rate‐limiting step of cerebral glucose metabolism occurred between 28°C and 32°C. To investigate the possible involvement of temperature‐dependent membrane phase transitions, brain perfusions were performed with a medium containing ethanol which is known to increase membrane fluidity. Perfusion of the mouse brain with a fluid containing 87 m m ethanol (400 mg/dl) resulted in decreases in the glucose utilization rates at 37°C but not at 28°C. At 28°C, ethanol produced an increase in glucose utilization in the medulla‐pons and in the cerebellum of perfused brains. In vivo rates of cerebral glucose utilization in two lines of mice selectively bred for differences in ethanol‐induced sleep time indicated that no differences in basal metabolism or following a 4‐g/kg dose of ethanol existed between the two lines. Interpretations of ethanol's differential effects on cerebral glucose utilization rates in the isolated perfused mouse brain at 28°C and 37°C are presented along with a discussion of the apparent discrepancies observed when in vivo and perfused brains are treated with equivalent concentrations of ethanol.