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Prolonged Feeding of Ethanol to the Young Growing Guinea Pig: 1, The Effect on Protein Synthesis in the Afterloaded Right Ventricle Measured in Vitro
Author(s) -
Schreiber Sidney S.,
Evans Carole D.,
Reff Francine,
Rothschild Marcus A.,
Oratz Murray
Publication year - 1982
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1982.tb04996.x
Subject(s) - guinea pig , ventricle , in vitro , ethanol , medicine , chemistry , biology , andrology , endocrinology , biochemistry
Newly weaned guinea pigs weighing approximately 300 g were fed normal laboratory diets with drinking water containing 5.5% ethanol as the sole source of liquid for periods of 8–11 weeks. Growth was continuous with this diet. After this period, hearts were removed from anesthetized animals and perfused for 3 hr in a perfusion system in which pressure may be induced in the right ventricle In the face of constant coronary flow. Protein synthesis, assayed from the incorporation of labeled lysine and phenylalanine, was compared to that in hearts from identically treated weight‐matched control animals who had been drinking water without ethanol. Protein synthesis in hearts from ethanoWrinking animals was decreased in the right ventricles exposed to normal pulmonary pressure, but was unchanged in the contracting but not working left ventricles. The data suggest that prolonged exposure even to low levels of ethanol in the growing animal may interfere with the cardiac protein synthetic response to the normal work stress.