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Ether Inhibition of Ethanol Metabolism in Isolated Rat Liver Parenchymal Cells
Author(s) -
Aune Hakon,
Stowell Allan R.,
Morland Jorg
Publication year - 1981
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1981.tb05359.x
Subject(s) - ethanol , acetaldehyde , diethyl ether , ether , chemistry , metabolism , ethanol metabolism , hepatocyte , alcohol , biochemistry , in vivo , chromatography , in vitro , organic chemistry , biology , microbiology and biotechnology
The effect of diethyl ether on ethanol metabolism was studied in isolated rat hepatocytes and ether was found to inhibit ethanol oxidation in a dose‐dependent manner. At ethanol concentrations of approximately 30 DIM, diethyl ether inhibited ethanol oxidation by approximately 58%, 40%, and 20% at ether concentrations of 30,20, and 10 mm, respectively. This inhibition was also seen at a low ethanol concentration (5.4 MM) and in pyruvate (5 mM)‐stimulated hepatocytes which exhibited increased rates of ethanol metabolism closer to in vivo rates. Accumulation of acetaldehyde from ethanol in cyanamide (400 μM)‐treated hepatocyte suspensions was also reduced by approximately 16% by 30 HIM ether, ft was concluded that inhibition of ethanol metabolism by diethyl ether might be of practical importance in studies of ethanol metabolism in ether anesthetized animals.