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Alcohol and Very Low Density Lipoprotein Synthesis and Secretion by Isolated Hepatocytes
Author(s) -
Lakshmanan M. R.,
Felver Michael E.,
Veech Richard L.
Publication year - 1980
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1980.tb04832.x
Subject(s) - very low density lipoprotein , hepatocyte , ethanol , leucine , stimulation , incubation , secretion , chemistry , low density lipoprotein , medicine , lipoprotein , protein biosynthesis , endocrinology , alcohol , biochemistry , biology , in vitro , amino acid , cholesterol
Labeled leucine can be used to measure accurately the rate of both total and secretory protein synthesis by isolated hepatocytes if at least 1 m M leucine is added to the incubation medium, even in the presence of 50 m M ethanol. Using this technique it was found that ethanol caused a significant inhibition of very low density lipoprotein (VLDL) as well as total protein synthetic rates in hepatocytes from both fed and fasted rats. In contrast, a single acute oral dose of ethanol to fasted rats caused within 4 hr a threefold stimulation in the rate of VLDL synthesis without affecting the total protein synthetic rate in the hepatocyte system.