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Acute Effect of Ethanol on Membranes of the Endoplasmic Reticulum and on Protein Synthesis in Rat Liver
Author(s) -
Murty Challakonda N.,
Verney Ethel,
Sidransky Herschel
Publication year - 1980
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1980.tb04797.x
Subject(s) - endoplasmic reticulum , microsome , polysome , membrane , biochemistry , chemistry , in vivo , albumin , ethanol , in vitro , biology , ribosome , rna , gene , microbiology and biotechnology
The acute effect of ethanol on the membranes of hepatic endoplasmic reticulum, on the in vitro protein‐synthetic activities of hepatic free and membrane‐bound polyribosomes and on the plasma proteins of rats fasted overnight was investigated. Ethanol (0.75 g/100 g body weight) was tube‐fed as a 50% (v/v) solution in saline 3 hr before sacrifice. Hepatic endoplasmic reticulum membranes from control and ethanol‐treated rats were compared using the following techniques: (1) lactoperoxidase‐catalyzed radioiodination of proteins and sodium dodecylsulfate‐polyacrylamide gel electrophoresis, and (2) measurement of 14 C‐choline incorporation into membranes. Hepatic microsomal membranes from ethanol‐treated rats incorporated in vitro less 12B I into total proteins (as well as into the 55,000 molecular weight proteins) and incorporated in vivo less 14 C‐choline into microsomal membranes than membranes of control rats. Ethanol administration inhibited in vivo incorporation of “C‐leucine or 14 C‐phenylalanine into liver protein and plasma albumin and globulin. The data also indicate that an acute dose of ethanol reduced the in vitro protein‐synthetic activity of hepatic membrane‐bound polyribosomes, while free polyribosomes were relatively unaffected.