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Estimation of Lipid Hydroperoxide Levels in Tropical Marine Macroalgae
Author(s) -
Kumari Puja,
Singh Ravindra Pal,
Bijo A. J.,
Reddy C. R. K.,
Jha Bhavanath
Publication year - 2012
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.2012.01208.x
Subject(s) - degree of unsaturation , biology , ulva lactuca , lactuca , tbars , botany , seawater , lipid peroxidation , polyunsaturated fatty acid , food science , chromatography , fatty acid , biochemistry , chemistry , ecology , antioxidant
The incipient levels of lipid hydroperoxides ( LHPO s) were determined in selected green, brown, and red macroalgae by the FOX assay using hydroperoxy HPLC mix. The LHPO s contents varied between the investigated species and showed relatively low values in this study. Among the greens, it varied from 12 ± 6.2 μg · g −1 ( C odium sursum ) to 31.5 ± 2.8 μg · g −1 ( U lva lactuca ), whereas in reds, from 5.7 ± 1.6 μg · g −1 ( G racilaria corticata ) to 46.2 ± 6 μg · g −1 ( S arconema filiforme ), and in browns, from 4.6 ± 4.4 μg · g −1 ( D ictyota bartayresiana ) to 79 ± 5.0 μg · g −1 ( S argassum tenerrimum ), on fresh weight basis. These hydroperoxides represented a minor fraction of total lipids and ranged from 0.04% ( S . swartzii ) to 1.1% ( S . tenerrimum ) despite being a rich source of highly unsaturated fatty acids. The susceptibility of peroxidation was assessed by specific lipid peroxidazibility ( SLP ) values for macroalgal tissues. The LHPO values were found to be independent of both the PUFA s contents and their degree of unsaturation ( DBI ), as evident from the PCA analysis. SLP values were positively correlated with the LHPO s and negatively with DBI . The FOX assay gave ≥20‐fold higher values for LHPO s as compared to the TBARS method for all the samples investigated in this study. Furthermore, U . lactuca cultured in artificial seawater ( ASW ) enriched with nutrients ( N , P , and NP ) showed a sharp decline in LHPO s contents relative to those cultured in ASW alone P ≤ 0.05. It is inferred from this study that the FOX assay is an efficient, rapid, sensitive, and inexpensive technique for detecting the incipient lipid peroxidation in macroalgal tissues.