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IN VITRO ANTIOXIDANT ACTIVITIES OF THE FERMENTED MARINE MICROALGA PAVLOVA LUTHERI (HAPTOPHYTA) WITH THE YEAST HANSENULA POLYMORPHA 1
Author(s) -
Qian ZhongJi,
Jung WonKyo,
Kang KyongHwa,
Ryu BoMi,
Kim SeKwon,
Je JaeYoung,
Heo SooJin,
Oh Chulhong,
Kang DoHyung,
Park Won Sun,
Choi IlWhan
Publication year - 2012
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.2012.01117.x
Subject(s) - biology , antioxidant , biochemistry , superoxide dismutase , lipid peroxidation , reactive oxygen species , yeast
Microalgae are major primary producers of organic matter in aquatic environments through their photosynthetic activities. Fermented microalga ( Pavlova lutheri Butcher) preparation (FMP) is the product of yeast fermentation by Hansenula polymorpha . It was tested for the antioxidant activities including lipid peroxidation inhibitory activity, free‐radical‐scavenging activity, inhibition of reactive oxygen species (ROS) on mouse macrophages (RAW264.7 cell), and inhibited myeloperoxidase (MPO) activity in human myeloid cells (HL60). FMP exhibited the highest antioxidant activity on free‐radical scavenging, inhibitory intracellular ROS, and inhibited MPO activity. MTT [3‐(4,5‐dimethyl‐2‐yl)‐2,5‐diphenyltetrazolium bromide] assay showed no cytotoxicity in mouse macrophages (RAW264.7 cell), human myeloid cells (HL60), and human fetal lung fibroblast cell line (MRC‐5). Furthermore, the antioxidative mechanism of FMP was evaluated by protein expression levels of antioxidant enzyme (superoxide dismutase [SOD] and glutathione [GSH]) using Western blot. The results obtained in the present study indicated that FMP is a potential source of natural antioxidant.