Premium
SEQUENCE AND STRUCTURAL ANALYSIS OF κ‐CARRAGEENAN‐DERIVED OLIGOSACCHARIDES BY TWO‐DIMENSIONAL NUCLEAR MAGNETIC RESONANCE 1
Author(s) -
Zhang Wei,
Liu Ming,
Hansen Poul Erik,
Yu Guangli,
Yang Bo,
Zhao Xia
Publication year - 2010
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.2010.00861.x
Subject(s) - heteronuclear molecule , nuclear magnetic resonance spectroscopy , carrageenan , oligosaccharide , two dimensional nuclear magnetic resonance spectroscopy , nuclear overhauser effect , sequence (biology) , heteronuclear single quantum coherence spectroscopy , spectroscopy , tetrasaccharide , hydrolysis , nuclear magnetic resonance , polysaccharide , crystallography , chemistry , stereochemistry , organic chemistry , biochemistry , physics , quantum mechanics
κ‐Carrageenan was hydrolyzed with mild hydrochloric acid and separated into a series of oligosaccharides, the sequences and structures of which were investigated by double‐quantum filtered correlation spectroscopy (DQF‐COSY), total correlation spectroscopy (TOCSY), heteronuclear multiple‐quantum coherence (HMQC), and heteronuclear multiple‐bond correlation (HMBC) techniques, respectively. The chemical structures and conformations of the individual sugar residues were identified, as well as the sequential connectivity of the oligosaccharides. The interresidue nuclear Overhauser effects (NOEs)/rotating frame Overhauser effects (ROEs) revealed an ordered helical structure of the carrageenan oligosaccharide chains. Therefore, a general two‐dimensional (2‐D) NMR methodology for the unambiguous sequence and structure analysis of κ‐carrageenan‐derived oligosaccharides was established in this study.