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PHENOTYPIC CHARACTERIZATION AND HYDROGEN PRODUCTION IN CHLAMYDOMONAS REINHARDTII Q B ‐BINDING D1‐PROTEIN MUTANTS UNDER SULFUR STARVATION: CHANGES IN CHL FLUORESCENCE AND PIGMENT COMPOSITION 1
Author(s) -
Faraloni Cecilia,
Torzillo Giuseppe
Publication year - 2010
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.2010.00857.x
Subject(s) - chlamydomonas reinhardtii , biology , mutant , xanthophyll , pigment , wild type , biochemistry , photosynthesis , photoinhibition , hydrogen production , botany , biophysics , photosystem ii , gene , chemistry , organic chemistry , catalysis
The effects of Q B ‐binding D1‐protein mutations on the phenotypic characteristics and on hydrogen production of sulfur‐deprived Chlamydomonas reinhardtii P. A. Dang. cultures were investigated. The mutation involved one (D240) or double (D239–40) amino‐acid deletions at positions 240 and 239–240, respectively, in the loop connecting helices D and E of the D1 protein. Phenotypic characterization of the mutants showed the following peculiarities as compared to the wildtype (WT): (i) a higher sensitivity to photoinhibition, (ii) a reduced amount of chl per dry weight and per cell, (iii) a higher respiration‐to‐photosynthesis ratio, (iv) a higher carbohydrate accumulation during the aerobic phase, and (v) a higher synthesis of xanthophyll‐cycle pigments. These differences were translated into a 12‐ to 18‐fold higher hydrogen biogas production.