Premium
ALLOPHYCOCYANIN FROM A LOCAL ISOLATE GEITLERINEMA SP. A28DM (CYANOBACTERIA): A SIMPLE AND EFFICIENT PURIFICATION PROCESS 1
Author(s) -
Parmar Asha,
Singh Niraj Kumar,
Madamwar Datta
Publication year - 2010
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.2009.00798.x
Subject(s) - allophycocyanin , cyanobacteria , phycobilisome , biology , phycobiliprotein , pigment , phycocyanin , cationic polymerization , size exclusion chromatography , sephadex , chromatography , yield (engineering) , botany , biochemistry , chemistry , genetics , organic chemistry , materials science , bacteria , enzyme , metallurgy
Allophycocyanin (APC) is the least‐studied cyanobacterial bile‐pigment invariably present within the phycobilisome core of cyanobacteria. In the present study, we describe a simple, cost‐effective, and reproducible method for the purification of APC from a local isolate, Geitlerinema sp. A28DM. The pigment was extracted from the algal biomass and precipitated with 0.25% aqueous solution of the highly aromatic cationic dye “ethodin.” The precipitated APC was then subjected to a single size‐exclusion chromatographic step using Sephadex G‐100. Pure cyanobacterial APC (C‐APC) ( A 652 / A 280 of 3.2) was obtained and characterized by its absorption spectrum with maximum at 652 nm and a shoulder at 620 nm, and by SDS‐PAGE, showing two bands with molecular masses of 15 and 17.5 kDa, corresponding to α and β subunits of the biliprotein. The final yield of C‐APC was 66% from its content in the crude extract. The procedure appears to be promising for wider applications and larger production of APC.