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IDENTIFICATION OF THE MINUS MATING‐TYPE SPECIFIC GENE MTD1 FROM GONIUM PECTORALE (VOLVOCALES, CHLOROPHYTA) 1
Author(s) -
Hamaji Takashi,
Ferris Patrick J.,
Nishii Ichiro,
Nozaki Hisayoshi
Publication year - 2009
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.2009.00744.x
Subject(s) - biology , chlamydomonas reinhardtii , mating type , chlamydomonas , chlorophyta , locus (genetics) , gene , genetics , gamete , chromosome , botany , algae , mutant , sperm
Gonium pectorale O. F. Müll. (Volvocales, Chlorophyta), a colonial 8‐ or 16‐cellular alga, is phylogenetically important as an intermediate form between isogametic unicellular Chlamydomonas and oogamous Volvox . We identified the mating‐type specific gene GpMTD1, from G . pectorale , the first homologue of Chlamydomonas reinhardtii MTD1 ( CrMTD1 ). The GpMTD1 gene was found to be present only in the minus mating‐type locus and was expressed specifically in the gametic phase as is the case for CrMTD1 , suggested to participate in development of the minus gametes. This gene is useful as a probe in analyzing the bacterial artificial chromosome (BAC) library for resolving genomic structures of the mating‐type loci in isogamous and oogamous colonial volvocaleans.