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EVALUATION OF ANTIOXIDANT DEFENSE RESPONSES TO LEAD STRESS IN HAPALOSIPHON FONTINALIS ‐339 1
Author(s) -
Zutshi Sunaina,
Choudhary Meenakshi,
Bharat Naveen,
Abdin Malik Zainul,
Fatma Tasneem
Publication year - 2008
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.2008.00542.x
Subject(s) - antioxidant , superoxide dismutase , catalase , glutathione reductase , glutathione , biology , toxicity , oxidative stress , apx , glutathione peroxidase , biochemistry , metal toxicity , enzyme , chemistry , organic chemistry
Lead (Pb) is a heavy metal and a potentially hazardous environmental pollutant. In this study, the potential of lead to induce oxidative stress in biological systems was assessed using the cyanobacterium Hapalosiphon fontinalis ‐339 as model test organism. The impact of lead toxicity on the cellular antioxidant system and the biochemical modulations that result in generation of antioxidant defense responses were also studied. To determine the effect of Pb toxicity, the test organism was grown in the presence of various concentrations (0.05, 0.10, 0.20, 0.40, 0.80, 1.0, 1.20, and 1.25 mg · L −1 ) of exogenous lead chloride (PbCl 2 ), and its effects on growth were observed in terms of the change in chl content. There was a significant increase in metal uptake by the alga with a concomitant decrease in growth. Lead stress appeared to significantly up‐regulate the levels of stress‐related antioxidant enzymes—such as superoxide dismutase (SOD), ascorbate peroxidase (APX), and glutathione reductase (GR)—while a decrease in catalase (CAT) levels was observed. In addition, the levels of nonenzymatic antioxidants, oxidized and total glutathione, were changed. Our results suggest the existence of a potent antioxidant defense machinery in H. fontinalis ‐339 and this organism can be employed to monitor lead toxicity in the environment.