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A TAXONOMIC STUDY OF HAFNIOMONAS (CHLOROPHYCEAE) BASED ON A COMPARATIVE EXAMINATION OF CULTURED MATERIAL 1
Author(s) -
Nakada Takashi,
Suda Shoichiro,
Nozaki Hisayoshi
Publication year - 2007
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.2007.00328.x
Subject(s) - biology , monophyly , chlorophyceae , botany , phylogenetic tree , taxonomy (biology) , contractile vacuole , ultrastructure , clade , algae , gene , chlorophyta , genetics , vacuole , cytoplasm
We studied the taxonomy of Hafniomonas species (Chlorophyceae) by LM and EM and DNA sequence analysis on eight culture strains, including six new isolates collected from various localities in Japan. The strains could be clearly delineated into five species, Hafniomonas reticulata (Korshikov) H. Ettl et Moestrup, H . montana (Geitler) H. Ettl et Moestrup, H . laevis Nakada, Suda et Nozaki sp. nov., H . conica (H. Ettl) Nakada et Nozaki stat. nov., and H . turbinea Nakada et Nozaki sp. nov., based on differences in cell shape, cell size, chloroplast surface, the number of contractile vacuoles, stigma ultrastructure, and muciferous vesicles at the periphery of protoplasts. Phylogenetic analyses of 18S rRNA and P700 chl a –apoprotein A2 ( psa B) gene sequences demonstrated that all Hafniomonas species constituted a robust clade in which four strains of H . reticulata formed a monophyletic group. These sequence data also showed sister relationships between H . montana and H . laevis , and between H . conica and H . turbinea . We also provided a Latin description of H . heom Nakada et Nozaki sp. nov., which was previously identified as H . reticulata .