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MOLECULAR CHARACTERIZATION OF A PHOSPHATE‐REGULATED CELL‐SURFACE PROTEIN FROM THE COCCOLITHOPHORID, EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE) 1
Author(s) -
Landry Dori M.,
Gaasterland Terry,
Palenik Brian P.
Publication year - 2006
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.2006.00247.x
Subject(s) - emiliania huxleyi , biology , phosphate , complementary dna , biochemistry , dna , genomic dna , intron , botany , gene , ecology , phytoplankton , nutrient
Emiliania huxleyi (Lohmann) Hay et Mohler is a cosmopolitan coccolithophorid that is known to be an excellent competitor for phosphate. A previous survey of cell‐surface proteins induced by phosphorus limitation in strain CCMP 374 yielded three abundant proteins. Using CCMP 1516, the strain chosen for genome sequence determination, we report the cDNA, genomic, and amino acid sequence of one cell‐surface phosphorus‐limitation induced protein and evidence that a second protein is highly similar. The introns within the genomic DNA encoding this cell‐surface protein as well as those defined by other phosphate‐regulated expressed sequence tags are analyzed. As these proteins are the most abundant cell‐surface proteins present under phosphorus limitation, they likely have a role in the ability of this organism to compete for phosphate.