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MOLECULAR PHYLOGENY OF CHLAMYDOMONAS REINHARDTII INFERRED FROM 3'UTR SEQUENCE COMPARISONS OF GAMETO‐LYSIN GENE
Publication year - 2001
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.2001.jpy37303-84.x
Subject(s) - biology , chlamydomonas reinhardtii , genetics , gene , restriction fragment length polymorphism , mating type , type (biology) , polymerase chain reaction , ecology , mutant
Matsuda, Y. 1 , Kubo, T. 2 , Abe, J. 2 & Saito, T. 31 Department of Biology, Faculty of Science, Kobe University, Nada, Kobe 657‐8501 Japan; 2 Department of Molecular Science, Graduate School of Science and Technology, Kobe University, Nada, Kobe 657‐8501 Japan; 3 Department of Applied Science, Faculty of Science, Okayama University of Science, Okayama 700‐0005 Japan In Chlamydomonas reinhardtii, wild‐type strains of three principal stock lines have been widely used for genetic, biochemical and morphological studies (Harris 1989). All have been identified as descendants of a mating pair (plus and minus) of clones derived from a zygospore isolated by G. M. Smith in 1945. Recently we noticed that there are several sequence differences between gametolysin cDNAs of 11‐32b plus and C‐9 minus strains. A clear RFLP was found between the two mating‐types when the 3'UTR was used as a probe to hybridize with genomic DNAs restricted with Sal I. The 3'UTR differed by the deletion of 12 nucleotides in the minus DNA. We therefore obtained representative strains of all three descendant lines of Smith's isolate and examined the RFLP and 3'UTR. All the plus strains of the Sager line (21gr, C‐8) and the Cambridge line (UTEX90, 11‐32b, C‐239) had the long (L)‐type 3'UTR, while the minus strains of the two lines (6145c, C‐9, UTEX89, 11‐32a, C‐238) had the short (S)‐type one. As for the Ebersold/Levine line stocks, both plus (137C, CC‐125, CC‐620) and minus (137C, CC‐124, CC‐621) strains had only the L‐type 3'UTR. We also examined 9 wild‐type strains of C. reinhardtii isolated from the wild (all obtained from CGC) and one strain of C. smithii (CC‐1373 plus). Surprisingly, the 3'UTR of C. smithii was 100% identical with the S‐type one of laboratory strains. A phylogenic tree indicates that the A‐type laboratory strains are included in a different clade from that of the B‐type/C. smithii.