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A PHYSICAL MAP OF THE CHLAMYDOMONAS REINHARDTII NUCLEAR GENOME
Publication year - 2001
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.2001.jpy37303-118.x
Subject(s) - contig , biology , genetics , gene mapping , chlamydomonas reinhardtii , genome , positional cloning , cloning (programming) , physical mapping , restriction map , computational biology , clone (java method) , molecular cloning , gene , chromosome , complementary dna , computer science , phenotype , mutant , plasmid , programming language
Silflow, C. D., Kathir, P., Brazelton, B. Haas, N. Nguyen, R. L. & Lefebvre, P. A. Department of Genetics, Cell Biology and Development and Department of Plant Biology, University of Minnesota, St. Paul, MN 55108 USA We will report an ongoing project to construct a physical map aligned with the genetic map of the Chlamydomonas genome. The project builds on a previously assembled molecular map consisting of more than 250 unique molecular markers placed on the 17 linkage groups of the genetic map with an average spacing of 4‐5 cM (400 ‐ 500 kb). Those markers have been used as hybridization probes to screen a BAC library constructed from C. reinhardtii DNA. The positive BAC clones have been assembled into “contigs” of approximately 130 kb by comparing a “fingerprint” of restriction fragments of each clone. The gaps between the mapped contigs are filled by screening the BAC library with unique probes derived from EST sequences and by “walking” from the ends of contigs. The use of the physical map to facilitate map‐based cloning of genes identified by mutation will be discussed.