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PARTIAL PURIFICATION OF FRUCTOSE 1,6‐BISPHOSPHATASE FROM THE MARINE MACR0ALGA BRYOPSIS CORTICULANS (CHLOROPHYCEAE) 1
Author(s) -
Yu Shukun
Publication year - 1989
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1989.tb00269.x
Subject(s) - biology , sephadex , fructose 1,6 bisphosphatase , size exclusion chromatography , chlorophyceae , fructose , biochemistry , water column , glycerol , chromatography , botany , algae , enzyme , chlorophyta , ecology , chemistry
Fructose 1,6‐bisphosphntase (FRPase, EC 3.1.3.11) partially purified from the marine green seauleed Bryopsis corticulans Setch exhibited a single active Peak on a Sephadex G‐200 column but two distinct peaks on a DEAE cellulose column. The two fractions shared the same molecular weight of 162,000 as revealed by gel filtration. The FBPase from Bryopsis was unstable in storage elen zlheii an appropriatr amount of 2‐mercaptoethnnol was present unless Mn 2+ and glycerol uere added in the buffer. Preliminary results indicate that the FBPase is of chloroplastic origin.