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DETECTION OF CHLOROPHYLLS c 1 , c 2 AND c 3 IN PIGMENT EXTRACTS OF PRYMNESIUM PARWM (PRYMNESIOPHYCEAE) 1
Author(s) -
Fawley Maruin W.
Publication year - 1989
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1989.tb00267.x
Subject(s) - chlorophyll , phaeodactylum tricornutum , biology , chlorophyll a , pigment , chlorophyll c , botany , chlorophyll b , algae , chlorophyll fluorescence , chemistry , organic chemistry
Three chlorophyll c‐type pigments were separated by reversed‐phase high Performance liquid chromatography and thin‐layer chromatography from pigment extracts of the prymnesiophyte, Prymnesium parvum Carter. Based on spectral characteristics, retention times, and comparison with reference pigments isolated from the diatom Phaeodactylum tricornutum Bohlin, two of these pigments were identijied as chlorophyll c 1 and c 2 . The other pigment was identified by its absorption spectrum and thin‐layer chromatography retention times as the newly described chlorophyll c 3 . However, in other prymnesiophytes so far examined, chlorophyll c 1 and chlorophyll c 3 were present with no chlorophyll c l . The discovery of chlorophyll c 3 with chlorophyll c 1 and chlorophyll c 3 in Prymnesium parvum therefore represents the first report of this combination of pigments in prymnesiophytes.