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PH‐DEPENDENCE OF CHLORTETRACYCLINE(CTC)‐INDUCED PLUG FORMATION IN NITELLA FLEXILIS (CHARACEAE) 1
Author(s) -
Foissner Ilse
Publication year - 1989
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1989.tb00128.x
Subject(s) - nitella , biology , biophysics , callose , cytochalasin b , botany , cell wall , biochemistry , cell
The formation of chlortetracycline(CTC)‐induced wall appositions (callose plugs) in Nitella flexilis (L.)Ag. was pH‐dependent in the range between 4.3‐8.3. Plug number and plug diameter increased with the pH of the CTC solution. At pH 4.3 plug formation was light‐dependent and occurred below the alkaline regions of the cell surface which form during photo synthetic assimilation of HCO 3 − . Inhibition of photosynthesis by 3–(3′,4′‐dichlorophenyl)‐1, 1‐dimethylurea prevented plug formation in the light. Dark‐treated cells could be induced to form plugs by raising the pH of the CTC solution. The formation of large but incomplete plugs in the presence of cytochalasin B is explained by the formation of numerous weak alkaline sites. I suggest that CTC enhances locally the Ca 2+ content at the cytoplasm near the plasmamembrane. The ionophoric character of CTC is probably more pronounced at high pH mainly because of a weaker binding with cations and a closer contact with the membrane.