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ISOLATION AND CHARACTERIZATION OF DOMINANT TUNICAMYCIN RESISTANCE MUTATIONS IN CHLAMYDOMONAS REINHARDTII (CHLOROPHYCEAE) 1
Author(s) -
Dutcher Susan K.,
Gibbons Wendy
Publication year - 1988
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1988.tb04238.x
Subject(s) - biology , chlamydomonas reinhardtii , chlorophyceae , tunicamycin , chlamydomonas , genetics , botany , gene , algae , mutant , chlorophyta , unfolded protein response
Seven independent mutations that confer resistance to the nucleoside antibiotic tunicamycin in the unicellular green alga Chlamydomonas reinhardtii were isolated in wild‐type diploid cells. Upon resolution to haploidy, all the mutations segregated as single mutations and were semi‐dominant when retested in heterozygous diploids. In addition, the TUN1‐3 allele affects the ability of cells to agglutinate during conjugation in the absence of tunicamycin. The seven mutations map to a single locus on linkage group VIII. These mutations may be useful as a selectable marker in transformation studies of Chlamydomonas and in studies of processes that require asparagine‐linked glycosylation.