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CELL DIVISION RATES OF EUCARYOTIC ALGAE MEASURED BY TRITIATED THYMIDINE INCORPORATION INTO DNA: COINCIDENT MEASUREMENTS OF PHOTOSYNTHESIS AND CELL DIVISION OF INDIVIDUAL SPECIES OF PHYTOPLANKTON ISOLATED FROM NATURAL POPULATIONS 1
Author(s) -
Rivkin Richard B.,
Voytek Mary A.
Publication year - 1986
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1986.tb04164.x
Subject(s) - biology , algae , thymidine , cell division , photosynthesis , phytoplankton , dna , botany , division (mathematics) , cell , genetics , ecology , nutrient , arithmetic , mathematics
The cell cycle marker event of DNA replication in eucaryotic algae was identified using 3 H‐Thymidine ( 3 H‐TdR) incorporation. The frequency of cells (F) within a population undergoing DNA replication was estimated and the cell division rate (μF) calculated. In laboratory cultures the rates of cell division calculated from changes in cell numbers (μN) and μF were similar. Dual labelling with 3 H‐TdR and NaH 14 CO 3 enabled rates of cell division and photosynthesis to be coincidently measured for individual species of algae. Using these single species radioisotope techniques, several distinct photosynthesis irradiance and cell division irradiance relationships were found for: (1) different species of phytoplankton isolated from the same sample, and (2) the same species isolated from different environments. These techniques allow the coupling between photosynthesis and cell division to be examined with high resolution for algae in situ.