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INCORPORATION OF TRITIATED THYMIDINE BY EUCARYOTIC MICROALGAE 1
Author(s) -
Rivkin Richard B.
Publication year - 1986
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1986.tb04163.x
Subject(s) - biology , thymidine , tritiated water , tritium , biochemistry , dna , physics , nuclear physics
The uptake and incorporation of tritiated thymidine ( 3 H‐TdR) by axenic laboratory cultures of marine diatoms and dinoflagellates was measured. 3 H‐TdR was incorporated into nucleic acids by all four algae examined during a two to six hour period prior to cytokinesis and not during other times of the cell cycle. Between 90‐95% of the 3 H label incorporated into (cold trichloroacetic acid insoluble) nucleic acids was recovered from DNA. Incorporation of 3 H‐TdR appears to accurately indicate the timing of DNA synthesis. The incorporation of 3 H‐TdR by eucaryotic algae during long term (24 h) incubations does not generally preclude using 3 H‐TdR uptake to estimate bacterial production and growth during short term incubations.