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31 P‐NMR STUDIES ON INORGANIC POLYPHOSPHATES IN MICROALGAE 1
Author(s) -
Okhama Tamiko,
Siebelt Friederike,
Furihata Kazuo,
Seto Haruo,
Miyachi Shigetoh,
Ohmori Masayuki
Publication year - 1986
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1986.tb02492.x
Subject(s) - polyphosphate , algae , hydrolysis , trichloroacetic acid , nuclear magnetic resonance spectroscopy , biology , phosphorus , nuclear magnetic resonance , nuclear chemistry , biochemistry , chemistry , botany , phosphate , organic chemistry , physics
Using “P nuclear magnetic resonance analysis, total inorganic polyphosphate in algae could be quantitatively estimated, For this purpose the algal suspension, which had been kept in cold trichloroacetic acid, was further treated with 6 mM EDTA, or the cells were kept in 2 N KOH containing 100 mM EDTA for 18 h at 37°C. These simple methods avoid hydrolysis of cellular inorganic polyphosphate and, therefore, are useful for the study of phosphorus metabolism in algae. The effects of these treatments on visualization of the signal for inorganic polyphosphate in nuclear magnetic resonance spectra were discussed in comparison with in vivo, ‘P nuclear magnetic resonance spectra of algae .