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THE NUCLEAR CELL CYCLE IN CHLAMYDOMONAS (CHLOROPHYCEAE) 1
Author(s) -
Coleman Annette W.
Publication year - 1982
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1982.tb03172.x
Subject(s) - biology , mitosis , cytokinesis , nuclear dna , cell division , dapi , cell cycle , chlamydomonas , dna , chlamydomonas reinhardtii , dna replication , chlorophyceae , cell nucleus , microbiology and biotechnology , dna synthesis , nucleus , gametogenesis , genetics , cell , botany , algae , chlorophyta , mitochondrial dna , gene , staining , mutant , embryo , cryopreservation
The timing of replication and division of the Chlamydomonas Ehrenberg nucleus in the vegetative cell cycle and at gametogenesis was examined, using fluorescence microspectrophotometry with two fluorochromes, mithramycin and 4′,6‐diamidino‐2‐phenylindole (DAPI). Under appropriate conditions, these bind specifically to DNA, and the fluorescence of the DNA fluorochrome complex is a quantitative measure of the DNA content. The alga is a haplont, which produces 2 n daughter cells at the time of vegetative reproduction; cytokinesis and daughter cell release lag behind karyokinesis. No nucleus was found to contain more than the 2c quantity of DNA. Hence daughter cell production proceeds by doubling of the nuclear DNA followed by karyokinesis, in a repetitive sequence. As reported previously for C. reinhardtii Dangeard, the gametes of C. moewusii Gerloff contain the 1c amount of nuclear DNA. Several conflicting interpretations of the cell cycle sequence proposed in the literature were resolved.