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ISOLATION AND PARTIAL CHARACTERIZATION OF NITRATE ASSIMILATION MUTANTS OF DUNALIELLA TERTIOLECTA (CHLOROPHYCEAE) 1
Author(s) -
Latorella A. Henry,
Bromberg Susan K.,
Lieber Kent,
Robinson James
Publication year - 1981
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1981.tb00841.x
Subject(s) - nitrate reductase , biology , chlorate , nitrate , chlorophyceae , nitrogen assimilation , nitrite , dunaliella , nitrite reductase , mutant , biochemistry , menadione , microbiology and biotechnology , botany , algae , enzyme , ecology , chlorophyta , chemistry , inorganic chemistry , gene
Fifteen nitrate assimilation‐deficient mutants of the euryhaline green alga, Dunaliella tertiolecta Butcher were selected by their chlorate resistance. Ten mutants, unable to grow on NO 3 − but able to grow on NO 2 − , had no detectable nitrate reductase activity. Five mutants, unable to grow on either NO 3 − or NO 2 − , had depressed levels of both nitrate and nitrite reductase. A method for assaying methyl viologen‐nitrate reductase in the presence of nitrite reductase is described.