Premium
13 C AND 15 N UPTAKE BY MARINE PHYTOPLANKTON. I. INFLUENCE OF NITROGEN SOURCE AND CONCENTRATION IN LABORATORY CULTURES OF DIATOMS 1
Author(s) -
Collos Yves,
Slawyk Gerd
Publication year - 1979
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1979.tb02983.x
Subject(s) - phaeodactylum tricornutum , nitrogen , biology , ammonium , nitrate , phytoplankton , nitrite , isotopes of nitrogen , nutrient , environmental chemistry , algae , redfield ratio , urea , ammonia , botany , carbon fibers , nuclear chemistry , biochemistry , ecology , chemistry , organic chemistry , materials science , composite number , composite material
Two species of marine diatoms, Skeletonema costatum (Grev.) Cleve and Phaeodactylum tricornutum Bohlin were grown in batch and continuous cultures on four different nitrogen compounds (nitrate, nitrite, ammonium, urea). Carbon and nitrogen uptake were measured simultaneously with the stable isotopes 13 C and 15 N. Nitrogen uptake generally increased with N concentration in the medium, but no clear difference existed between the N sources. Carbon fixation was decreased for up to 5 h following the addition of the N compound. Nitrite generally had the greatest inhibitory effect on C uptake. Carbon‐to‐nitrogen uptake ratios decreased with increasing dissolved N concentration, becoming lower than one in nutrient‐limited cultures. In contrast, batch cultures exhibited C:N uptake ratios greater than one. These effects are essentially short‐term and differ from long‐term influences of the N source on the cellular chemical composition.