Premium
MICROFLUOROMETRIC MEASUREMENT OF DNA IN EUDORINA ELEGANS AND E. CALIFORNICA (CHOROPHYCEAE) 1
Author(s) -
Lindley Kemp C.,
Doyle Gordon,
Anderson Ron
Publication year - 1979
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1979.tb00720.x
Subject(s) - biology , feulgen stain , dna , ploidy , fluorescence , somatic cell , microbiology and biotechnology , genetics , gene , optics , physics
The fluorescent dye 2,5‐bis (4′‐aminophenyl (1′))‐1,3,4‐oxidiwle (BAO) was compared to the Feulgen procedure as a method for quantitative comparison of DNA levels in two species of Eudorina. The BAO procedure proved to be specific and as quantitative as the Feulgen procedure, and was more convenient and rapid. The DNA level of the nuclei of gonidial cells of Eudorina elegans Ehrenberg and E. californica (Shaw) Goldstein doubled prior to each division. The DNA level of the somatic, usually non‐dividing nuclei of E. californica did not vary from the IC (haploid) level.