Premium
MEASUREMENTS OF PHYTOPLANKTON‐PROTEIN CONTENT WITH THE HEATED BIURET‐FOL1N ASSAY 1 , 2
Author(s) -
Dorsey Thomas E.,
McDonald Paul,
Roels Oswald A.
Publication year - 1978
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1978.tb02443.x
Subject(s) - biuret test , algae , biology , chromatography , volume (thermodynamics) , nitrogen , zoology , biochemistry , botany , urea , chemistry , physics , organic chemistry , quantum mechanics
The heated biuret‐Folin method for determining protein consistently measures 90% of the total nitrogen of filtered algae samples as protein‐N without the need of mechanical disruption as long as the heating period in biuret is 100 min at 100 C. Data indicate this protein assay measures total protein on all species tried and for naturally occurring mixtures of species plus detritus. Dilute algal suspensions with as little as 0.05 μg‐atom particulate protein N.liter‐1can he concentrated by fltration on glass fiber filters to 1.0 μg‐atom particulate protein‐N per filter, the optimal amount of sample for a 5 ml volume of biuret. The filtered algae samples can be stored for several weeks frozen before assaying, if necessary .