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MOLECULAR, ENZYMATIC AND ULRASTRUCURE CHARACTERIZATION OF THE PYRENOID OF THE SCALY GREEN MONAD MICROMONAS SQUAMATA 1, 2
Author(s) -
Salisbury Jeffrey L.,
Floyd Gary L.
Publication year - 1978
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1978.tb00313.x
Subject(s) - pyrenoid , biology , biochemistry , chloroplast , gene
Pyrenoid material of micromonas squamata Manton & Parke was obtained free of cell and subcellular particle conamination by differential centrifugation of brei from osmoically lysed cells. The isolated pyrenoid particles were characterized by transmission and scanning electron microscopy. Sodium dodecyl sulfate polyacrylamide gel electrophoresis of pyrenoid extracts revealed a compled polypeptide composition with major components of 12, 54 and 66 kilodalton mol wt. Whole pyrenoids possessed the enzymatic properties of ribulose diphosphate carboxylase and fixed carbon dioxide with specific activity 10 times greaer than that of a pyrenoid‐free high speed supenaant fracion of cell brei. Energy dispersive X‐ray microanalysis revealed he presence of copper in masses of cryo‐impacted pyrenoid material. Ultrastrucural cytochemistry was employed o determine he chemical nature of the reserve carbohydrate shell. Also, the pyrenoid of the intact cell was characterized by transmission electron microscopy.