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ALCIAN BLUE: A QUANTITATIVE AQUEOUS ASSAY FOR ALGAL ACID AND SULFATED POLYSACCHARIDES 1
Author(s) -
Ramus J.
Publication year - 1977
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1977.tb02939.x
Subject(s) - alginic acid , polysaccharide , aqueous solution , sulfation , carrageenan , sulfate , cationic polymerization , chromatography , biology , agar , nuclear chemistry , biochemistry , chemistry , organic chemistry , bacteria , genetics
Alcian Blue, a cationic copper phthalocyanine dye, complexes with the anionic carboxyl and half‐ester sulfate groups of acidic algal polysaccharide in aqueous solution to form an insoluble precipitate. The quantity of dye removed from solution is proportional to the quantity of polyanion in solution, and this principle forms the basis for the quantitative determination of acid and/or sulfated algal polysaccharides. The assay is linear between 0 and 100 μg/ml agar, alginic acid, carrageenan, pectin and Porphyridium aerugineum Geit. polysaccharide. In addition, the technique is used to determine the anion density of acid polysaccharides on a molar or weight equivalency basis.