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EXTRACELLULAR LYSIS OF THE BLUEGREEN ALGA PHORMIDIUM LURIDUM BY BDELLOVIBRIO BACTERIOVORUS 1
Author(s) -
Burnham Jeffrey C.,
Stetak Thomas,
Locher Gregory
Publication year - 1976
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1976.tb02849.x
Subject(s) - bdellovibrio , lysis , biology , extracellular , intracellular , microbiology and biotechnology , lytic cycle , photosynthesis , bacteria , cyanobacteria , protoplast , botany , biochemistry , virology , virus , genetics
SUMMARY When either cells of the bacterium, Bdellovibrio bacteriovorus (Stolp & Starr), strain 15143, or a heat‐resistant lytic factor derived from these cells is added to viable cultures of Phormidium luridum var. olivacea Boresch all the algal cells underwent gradual lysis. This effect was obtained with a mean initial bdellovibrio:algal cell ratio of 7.5:1. When P. luridum was mixed with the bdellovibrio cultures the algal chlorophyll content showed an 8‐fold decrease. Concomitantly, this interspecies interaction caused, a 75% inhibition of algal photosynthesis after‐4 h. Heat, treatment of the B. bacteriovorus culture supernatant fluid increased its ability to inhibit photosynthesis approximately 14%. Light, microscopy showed pale granules and intracellular spaces to form in the P. luridum within 16 h after adding the bdellovibrio lytic factor. Subsequent morphological changes included the development of large intracellular spaces, intercellular spaces, spheroplast formation and finally Complete lysis of the algal cells.