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THE LIFE HISTORY OF COLEOCHAETE SCUTATA (CHLOROPHYCEAE) STUDIED BY A FEULGEN MICROSPECTROPHOTOMETRIC ANALYSIS OF THE DNA CYCLE 1, 2
Author(s) -
Hopkins Archibald W.,
McBride Gordon E.
Publication year - 1976
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1976.tb02822.x
Subject(s) - biology , feulgen stain , thallus , ploidy , nuclear dna , gamete , mitosis , dna , dna synthesis , sexual reproduction , botany , life history , chlorophyceae , cell division , algae , sperm , genetics , cell , ecology , mitochondrial dna , chlorophyta , gene
SUMMARY The life history of Coleochaete scutata Bréb. was analyzed by Feulgen microspectrophotometry, a technique measuring DNA content in individual nuclei. By correlating nuclear DNA content with morphological structures or stages in the life history, changes in ploidy level are revealed. The microspectrophotometric study confirmed the earlier reports of a haploid vegetative thallus with mitotic division restricted primarily to the margin of the thallus. In the mitotic cycle the G 1 (pre‐synthesis) phase is longer in duration than the synthesis find G 2 (post‐synthesis) phases. Oogamous sexual reproduction results in resistant oospores which attain DNA levels of 2C 8C (1C being the DNA level of gamete nuclei).