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SOME CHARACTERISTICS OF THE SPERM ATTRACTANT FROM OEDOGONIUM CARDIACUM 1
Author(s) -
Machlis Leonard,
Hill Gerry G. C.,
Steinback Katherine E.,
Reed Walfred
Publication year - 1974
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1974.tb02698.x
Subject(s) - pigment , biology , size exclusion chromatography , ultrafiltration (renal) , bioassay , chromatography , sperm , filtration (mathematics) , absorption (acoustics) , algae , botany , biochemistry , chemistry , ecology , materials science , organic chemistry , statistics , mathematics , composite material , enzyme
SUMMARY A bioassay for the sperm attractant from Oedogonium cardiacum was developed. The attractant was confirmed to be highly water soluble, destroyed by heating to 100 C and by dilute acid or alkali. It was fractionated by gel filtration with the attractant accompanied by yellow pigments. It has it molecular weight between 500 and about 1500, based on gel filtration, ultrafiltration, and dialysis. Freezing changes this to both larger and smaller molecules. It appears to have cationic groups. A paper chromatographic separation was found that divided the pigment into at least 3 major bands, one of which was closely associated with the attractant. An absorption spectrum of this pigment showed 1 peak in the visible spectrum at 417 mμ and 3 well‐defined peaks in the ultraviolet region. The lack of a peak at 280 mμ indicates the attractant is not a polypeptide.