Premium
DNA SYNTHESIS IN CRYPTOTHECODINIUM COHNII 1
Author(s) -
Franker Colin K.,
Sakhrani Lahki M.,
Prichard Candyce D.,
Lawden Carol A.
Publication year - 1974
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.1529-8817.1974.tb02683.x
Subject(s) - biology , elution , dna , dna synthesis , phase (matter) , biophysics , microbiology and biotechnology , chromatography , biochemistry , physics , chemistry , quantum mechanics
SUMMARY Thermal denaturation profiles of 32 P‐labeled DNA obtained from synchronous cultures of Cryptothecodinium cohnii were examined by elution from hydroxyapatite over a temperature range of 65 to 99 C. The profile of uniformly labeled DNA from this organism shows at least 4 discrete components which elute after a temperature of 83 C is reached. These components from the “high melt” region of the profile appear to be synthesized during the latter one‐third of the S phase in synchronous culture.